Genotyping and primer details


Genotyping and primer details for 038F09 - At5g58660
Line ID 038F09
Locus At5g58660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23702164 of line 038F09 Chr5:23702164
   
Insertion allele amplicon 8474 - KR30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR30: 5'-TCTAGAGAATCACGGAGTACCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23701682
   
wt amplicon (over InsPos) 43wn - KR30
wt amplicon start Chr5:23701682
wt amplicon end Chr5:23702811
wt amplicon size 1130 bp
Locus specific primer KR30: 5'-TCTAGAGAATCACGGAGTACCGTT-3'
2nd locus specific primer 43wn: 5'-GAACAAGTTGATGTATTCCAGCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11