Genotyping and primer details


Genotyping and primer details for 038G12 - At5g50550
Line ID 038G12
Locus At5g50550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20578222 of line 038G12 Chr5:20578222
   
Insertion allele amplicon 8474 - 49mv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 49mv: 5'-CACATGCATCAGTTAAAAGCCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20577584
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11