Genotyping and primer details


Genotyping and primer details for 039B02 - At4g21210
Line ID 039B02
Locus At4g21210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11307865 of line 039B02 Chr4:11307865
   
Insertion allele amplicon 3144 - FL81
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FL81: 5'-TTTTTACTAAACCAAACCAAACGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11307513
   
Insertion allele amplicon 8474 - MM26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MM26: 5'-AAACAAAGTCTTGGGAAGATCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11308210
   
wt amplicon (over InsPos) FL81 - MM26
wt amplicon start Chr4:11307513
wt amplicon end Chr4:11308210
wt amplicon size 697 bp
Locus specific primer MM26: 5'-AAACAAAGTCTTGGGAAGATCAAC-3'
2nd locus specific primer FL81: 5'-TTTTTACTAAACCAAACCAAACGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11