Genotyping and primer details


Genotyping and primer details for 039F11 - At5g66320
Line ID 039F11
Locus At5g66320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26496232 of line 039F11 Chr5:26496232
   
Insertion allele amplicon 8474 - 01uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01uj: 5'-GGTGTCACTTTTAGTTTGGTCCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26495795
   
wt amplicon (over InsPos) 27vg - 01uj
wt amplicon start Chr5:26495795
wt amplicon end Chr5:26496551
wt amplicon size 757 bp
Locus specific primer 01uj: 5'-GGTGTCACTTTTAGTTTGGTCCAT-3'
2nd locus specific primer 27vg: 5'-CCAAGAAGCATAAGAAAAGGTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11