Genotyping and primer details


Genotyping and primer details for 040B02 - At1g15280
Line ID 040B02
Locus At1g15280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5253583 of line 040B02 Chr1:5253583
   
Insertion allele amplicon 3144 - 03dl
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 03dl: 5'-AGGGTTGGATTTTTCAAAGTAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5253040
   
wt amplicon (over InsPos) 03eh - 03dl
wt amplicon start Chr1:5253040
wt amplicon end Chr1:5253864
wt amplicon size 825 bp
Locus specific primer 03dl: 5'-AGGGTTGGATTTTTCAAAGTAGGT-3'
2nd locus specific primer 03eh: 5'-ACGAGAGTTTTGAGACCCATTAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11