Genotyping and primer details


Genotyping and primer details for 040D01 - At5g16320
Line ID 040D01
Locus At5g16320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5344522 of line 040D01 Chr5:5344522
   
Insertion allele amplicon 8474 - 06kk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06kk: 5'-AAATCAGATTCTACAGGAAGCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5343989
   
wt amplicon (over InsPos) 06lb - 06kk
wt amplicon start Chr5:5343989
wt amplicon end Chr5:5344851
wt amplicon size 863 bp
Locus specific primer 06kk: 5'-AAATCAGATTCTACAGGAAGCCAA-3'
2nd locus specific primer 06lb: 5'-CTTAATCAAACCAATTCCGTCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11