Genotyping and primer details


Genotyping and primer details for 041B01 - At5g10860
Line ID 041B01
Locus At5g10860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3429573 of line 041B01 Chr5:3429573
   
Insertion allele amplicon 8474 - PA16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PA16: 5'-ATCTGGATTCGAGAGCACAACTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3429996
   
wt amplicon (over InsPos) 10rd - PA16
wt amplicon start Chr5:3429136
wt amplicon end Chr5:3429996
wt amplicon size 861 bp
Locus specific primer PA16: 5'-ATCTGGATTCGAGAGCACAACTAT-3'
2nd locus specific primer 10rd: 5'-CACAACAGGGAACTCTTTTCTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11