Genotyping and primer details


Genotyping and primer details for 042B09 - At1g16840
Line ID 042B09
Locus At1g16840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5763314 of line 042B09 Chr1:5763314
   
Insertion allele amplicon 3144 - 07ft
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 07ft: 5'-ATTTCACGTCTTTTCACTTGCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5762884
   
Insertion allele amplicon 8474 - 76ww
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 76ww: 5'-CAAAACTCAAAATTACAGCGTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5764060
   
wt amplicon (over InsPos) 07ft - 01dr
wt amplicon start Chr1:5762884
wt amplicon end Chr1:5763866
wt amplicon size 983 bp
Locus specific primer 01dr: 5'-GGGGAGATCTGTACGAGATTGATA-3'
2nd locus specific primer 07ft: 5'-ATTTCACGTCTTTTCACTTGCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11