Genotyping and primer details


Genotyping and primer details for 043E04 - At5g51640
Line ID 043E04
Locus At5g51640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20976964 of line 043E04 Chr5:20976964
   
Insertion allele amplicon 8474 - 01fx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01fx: 5'-GGACTCCATCTGATTACGAGCTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12971467
   
wt amplicon (over InsPos) 02hi - 01fx
wt amplicon start Chr4:12971467
wt amplicon end Chr5:20977332
wt amplicon size 983 bp
Locus specific primer 01fx: 5'-GGACTCCATCTGATTACGAGCTAC-3'
2nd locus specific primer 02hi: 5'-TGTTGTTTCCTCGAAAAGTTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11