Genotyping and primer details


Genotyping and primer details for 045E01 - At5g04170
Line ID 045E01
Locus At5g04170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1145618 of line 045E01 Chr5:1145618
   
Insertion allele amplicon 8474 - 39fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39fr: 5'-AGCTCCTTATCATCAATAAAGCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1146201
   
wt amplicon (over InsPos) 24go - 39fr
wt amplicon start Chr5:1145329
wt amplicon end Chr5:1146201
wt amplicon size 873 bp
Locus specific primer 39fr: 5'-AGCTCCTTATCATCAATAAAGCCA-3'
2nd locus specific primer 24go: 5'-CCCGCTAATTATAGTGTCGGTTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11