Genotyping and primer details


Genotyping and primer details for 047E09 - At3g44320
Line ID 047E09
Locus At3g44320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:15994784 of line 047E09 Chr3:15994784
   
Insertion allele amplicon 8474 - DC82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DC82: 5'-GGTGAAATAATGACACTTCCACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:15988538
   
Insertion allele amplicon 8474 - FL95
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL95: 5'-TTAGCTGCTGTTGTTGGAGTCTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:15994352
   
Insertion allele amplicon 8474 - RH04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RH04: 5'-GGTGAAATAATGACACTTCCACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:15988538
   
wt amplicon (over InsPos) FL95 - RH04
wt amplicon start Chr3:15988538
wt amplicon end Chr3:15994352
wt amplicon size 821 bp
Locus specific primer RH04: 5'-GGTGAAATAATGACACTTCCACCT-3'
2nd locus specific primer FL95: 5'-TTAGCTGCTGTTGTTGGAGTCTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11