Genotyping and primer details


Genotyping and primer details for 047E12 - At1g53560
Line ID 047E12
Locus At1g53560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19984922 of line 047E12 Chr1:19984922
   
Insertion allele amplicon 3144 - FP17
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FP17: 5'-AAGTGGTGAAATTTTGTTGTACCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19984439
   
Insertion allele amplicon 8474 - KM15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KM15: 5'-CTCTTCCAGAAGCAAAAGCTTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19985443
   
wt amplicon (over InsPos) FP17 - KM15
wt amplicon start Chr1:19984439
wt amplicon end Chr1:19985443
wt amplicon size 1005 bp
Locus specific primer KM15: 5'-CTCTTCCAGAAGCAAAAGCTTAAA-3'
2nd locus specific primer FP17: 5'-AAGTGGTGAAATTTTGTTGTACCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11