Genotyping and primer details


Genotyping and primer details for 049H07 - At4g36870
Line ID 049H07
Locus At4g36870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17370778 of line 049H07 Chr4:17370778
   
Insertion allele amplicon 3144 - FM06
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FM06: 5'-GAAGAAGAACAAACTTGGGAACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17370433
   
Insertion allele amplicon 8474 - JU15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JU15: 5'-TATGTCGAAAGAGTTCACAACCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17371510
   
wt amplicon (over InsPos) FM06 - JU15
wt amplicon start Chr4:17370433
wt amplicon end Chr4:17371510
wt amplicon size 1077 bp
Locus specific primer JU15: 5'-TATGTCGAAAGAGTTCACAACCAT-3'
2nd locus specific primer FM06: 5'-GAAGAAGAACAAACTTGGGAACAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11