Genotyping and primer details


Genotyping and primer details for 050C07 - At2g20270
Line ID 050C07
Locus At2g20270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8739030 of line 050C07 Chr2:8739030
   
Insertion allele amplicon 8474 - 02ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02ve: 5'-ACAAGAAAGAACAAATGGTTTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8738593
   
wt amplicon (over InsPos) 74wa - 02ve
wt amplicon start Chr2:8738593
wt amplicon end Chr2:8739557
wt amplicon size 965 bp
Locus specific primer 02ve: 5'-ACAAGAAAGAACAAATGGTTTTGG-3'
2nd locus specific primer 74wa: 5'-GCAATCTCTTTCTCCATGTTAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11