Genotyping and primer details


Genotyping and primer details for 051A05 - At3g54400
Line ID 051A05
Locus At3g54400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20142442 of line 051A05 Chr3:20142442
   
Insertion allele amplicon 8474 - CW48
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW48: 5'-GTTCGTATTTCGGTTTTGGTTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20141809
   
wt amplicon (over InsPos) 01dk - CW48
wt amplicon start Chr3:20141809
wt amplicon end Chr3:20142882
wt amplicon size 1074 bp
Locus specific primer CW48: 5'-GTTCGTATTTCGGTTTTGGTTTTA-3'
2nd locus specific primer 01dk: 5'-CAACACCTTGGGTAATGAGTTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11