Genotyping and primer details


Genotyping and primer details for 051G05 - At5g49980
Line ID 051G05
Locus At5g49980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20335424 of line 051G05 Chr5:20335424
   
Insertion allele amplicon 3144 - 27ke
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 27ke: 5'-TAAGCTCTGGACAGTTCTCTGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20334944
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11