Genotyping and primer details


Genotyping and primer details for 053D12 - At5g27330
Line ID 053D12
Locus At5g27330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9633037 of line 053D12 Chr5:9633037
   
Insertion allele amplicon 8474 - 05kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05kh: 5'-ATCAAGCCTAAGTGGAGACAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9632676
   
wt amplicon (over InsPos) 02na - 05kh
wt amplicon start Chr5:9632676
wt amplicon end Chr5:9633394
wt amplicon size 719 bp
Locus specific primer 05kh: 5'-ATCAAGCCTAAGTGGAGACAAAAA-3'
2nd locus specific primer 02na: 5'-AGTCATTGAGAAACGAAAAGGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11