Genotyping and primer details


Genotyping and primer details for 053G06 - At1g35510
Line ID 053G06
Locus At1g35510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:13074447 of line 053G06 Chr1:13074447
   
Insertion allele amplicon 8474 - FM16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FM16: 5'-AAGGGAGAATGGTCACTAATGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13074879
   
Insertion allele amplicon 8474 - MR96
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR96: 5'-GGGTGAGAATGTTGAAATGTTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13073967
   
wt amplicon (over InsPos) FM16 - MR96
wt amplicon start Chr1:13073967
wt amplicon end Chr1:13074879
wt amplicon size 913 bp
Locus specific primer MR96: 5'-GGGTGAGAATGTTGAAATGTTTTT-3'
2nd locus specific primer FM16: 5'-AAGGGAGAATGGTCACTAATGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11