Genotyping and primer details


Genotyping and primer details for 053G08 - At1g18150
Line ID 053G08
Locus At1g18150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6245118 of line 053G08 Chr1:6245118
   
Insertion allele amplicon 3144 - DL94
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer DL94: 5'-TTACCGAGAGGTAACAAACAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6245508
   
Insertion allele amplicon 8474 - FI20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FI20: 5'-TAAGCAAACTTCATCGTCATGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6244746
   
wt amplicon (over InsPos) DL94 - FI20
wt amplicon start Chr1:6244746
wt amplicon end Chr1:6245508
wt amplicon size 763 bp
Locus specific primer FI20: 5'-TAAGCAAACTTCATCGTCATGTTC-3'
2nd locus specific primer DL94: 5'-TTACCGAGAGGTAACAAACAAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11