Genotyping and primer details


Genotyping and primer details for 054G07 - At1g12070
Line ID 054G07
Locus At1g12070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4079805 of line 054G07 Chr1:4079805
   
Insertion allele amplicon 3144 - EL01
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer EL01: 5'-TAAATCATGTCGAATCAAATCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4080196
   
Insertion allele amplicon 3144 - FI21
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FI21: 5'-TAGAGCCTTCTTTGAGAGTGAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4079454
   
wt amplicon (over InsPos) FI21 - EL01
wt amplicon start Chr1:4079454
wt amplicon end Chr1:4080196
wt amplicon size 743 bp
Locus specific primer EL01: 5'-TAAATCATGTCGAATCAAATCCAA-3'
2nd locus specific primer FI21: 5'-TAGAGCCTTCTTTGAGAGTGAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11