Genotyping and primer details


Genotyping and primer details for 055A05 - At2g24645
Line ID 055A05
Locus At2g24645 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10481103 of line 055A05 Chr2:10481103
   
Insertion allele amplicon 8474 - 04sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04sm: 5'-GTTGGTATCTTTATTTTGTGCGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10480474
   
wt amplicon (over InsPos) 01sz - 04sm
wt amplicon start Chr2:10480474
wt amplicon end Chr2:10481483
wt amplicon size 1010 bp
Locus specific primer 04sm: 5'-GTTGGTATCTTTATTTTGTGCGGT-3'
2nd locus specific primer 01sz: 5'-AAGACGTGGAGAGATGATTCTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11