Genotyping and primer details


Genotyping and primer details for 055E09 - At2g29050
Line ID 055E09
Locus At2g29050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12479063 of line 055E09 Chr2:12479063
   
Insertion allele amplicon 8474 - DL05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DL05: 5'-AAAAGCACTAAAGAGGTGATCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12479607
   
wt amplicon (over InsPos) 06hn - DL05
wt amplicon start Chr2:12478636
wt amplicon end Chr2:12479607
wt amplicon size 972 bp
Locus specific primer DL05: 5'-AAAAGCACTAAAGAGGTGATCCAA-3'
2nd locus specific primer 06hn: 5'-GTGTCTCTGAGTTTGAAATGTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11