Genotyping and primer details


Genotyping and primer details for 055F01 - At1g80610
Line ID 055F01
Locus At1g80610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30303044 of line 055F01 Chr1:30303044
   
Insertion allele amplicon 8474 - 03ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03ve: 5'-GTAGCTTGTGGGTATTTTGGTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30303435
   
wt amplicon (over InsPos) 73wa - 03ve
wt amplicon start Chr1:30302434
wt amplicon end Chr1:30303435
wt amplicon size 1002 bp
Locus specific primer 03ve: 5'-GTAGCTTGTGGGTATTTTGGTCAT-3'
2nd locus specific primer 73wa: 5'-ACCCCAAACTAGTGTCACGTATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11