Genotyping and primer details


Genotyping and primer details for 055F02 - At3g09820
Line ID 055F02
Locus At3g09820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3013682 of line 055F02 Chr3:3013682
   
Insertion allele amplicon 3144 - KM24
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KM24: 5'-ACTGGGTACTTCTTCACTTTTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3014137
   
wt amplicon (over InsPos) 12sl - KM24
wt amplicon start Chr3:3013184
wt amplicon end Chr3:3014137
wt amplicon size 954 bp
Locus specific primer KM24: 5'-ACTGGGTACTTCTTCACTTTTCCA-3'
2nd locus specific primer 12sl: 5'-CTTGGTGGAGAAAGGTCAGTTTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11