Genotyping and primer details


Genotyping and primer details for 055H09 - At4g38390
Line ID 055H09
Locus At4g38390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17976497 of line 055H09 Chr4:17976497
   
Insertion allele amplicon 8474 - FM33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FM33: 5'-CTCTTTGAAGTTGAAGAAAAGGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17976047
   
Insertion allele amplicon 8474 - MR98
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR98: 5'-GGTGGATATTCTTGACTGCTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17977007
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11