Genotyping and primer details


Genotyping and primer details for 057E08 - At3g13930
Line ID 057E08
Locus At3g13930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4599541 of line 057E08 Chr3:4599541
   
Insertion allele amplicon 8474 - 06de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06de: 5'-GGTGCATCTTACTTCTTTTCTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4599131
   
wt amplicon (over InsPos) 05dy - 06de
wt amplicon start Chr3:4599131
wt amplicon end Chr3:4599847
wt amplicon size 717 bp
Locus specific primer 06de: 5'-GGTGCATCTTACTTCTTTTCTTGC-3'
2nd locus specific primer 05dy: 5'-AAACTTCAACGGATAAAGTGGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11