Genotyping and primer details


Genotyping and primer details for 058F03 - At4g27940
Line ID 058F03
Locus At4g27940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13904748 of line 058F03 Chr4:13904748
   
Insertion allele amplicon 8474 - 21af
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21af: 5'-ATTTCCCAGATGAATCAAGACAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13905042
   
wt amplicon (over InsPos) 09vu - 21af
wt amplicon start Chr4:13903906
wt amplicon end Chr4:13905042
wt amplicon size 1137 bp
Locus specific primer 21af: 5'-ATTTCCCAGATGAATCAAGACAAC-3'
2nd locus specific primer 09vu: 5'-CTTCACCACCAAATTACTTCCCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11