Genotyping and primer details


Genotyping and primer details for 058G12 - At4g28680
Line ID 058G12
Locus At4g28680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14157305 of line 058G12 Chr4:14157305
   
Insertion allele amplicon 8474 - 07rn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07rn: 5'-ACTGCATCAACCAAATTTTTAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14156950
   
wt amplicon (over InsPos) 45si - 07rn
wt amplicon start Chr4:14156950
wt amplicon end Chr4:14157698
wt amplicon size 749 bp
Locus specific primer 07rn: 5'-ACTGCATCAACCAAATTTTTAACC-3'
2nd locus specific primer 45si: 5'-CATCTCATTCAACATATTTCACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11