Genotyping and primer details


Genotyping and primer details for 060D03 - At2g25355
Line ID 060D03
Locus At2g25355 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10802237 of line 060D03 Chr2:10802237
   
Insertion allele amplicon 8474 - 03et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03et: 5'-TGATGATGGTTCAGATTGAAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10802670
   
wt amplicon (over InsPos) 80vs - 03et
wt amplicon start Chr2:10801312
wt amplicon end Chr2:10802670
wt amplicon size 1359 bp
Locus specific primer 03et: 5'-TGATGATGGTTCAGATTGAAGAAG-3'
2nd locus specific primer 80vs: 5'-CTACCTCTCAGTTCTGCTTTTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11