Genotyping and primer details


Genotyping and primer details for 062H01 - At1g03365
Line ID 062H01
Locus At1g03365 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:828143 of line 062H01 Chr1:828143
   
Insertion allele amplicon 8474 - AG05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AG05: 5'-TGTTTGAGGATTTACAACAGCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:827681
   
Insertion allele amplicon 8474 - MF06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MF06: 5'-AGACTTCTTTTCTGAAGCTGATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:828516
   
wt amplicon (over InsPos) AG05 - MF06
wt amplicon start Chr1:827681
wt amplicon end Chr1:828516
wt amplicon size 836 bp
Locus specific primer MF06: 5'-AGACTTCTTTTCTGAAGCTGATGG-3'
2nd locus specific primer AG05: 5'-TGTTTGAGGATTTACAACAGCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11