Genotyping and primer details


Genotyping and primer details for 065E10 - At1g13280
Line ID 065E10
Locus At1g13280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4548445 of line 065E10 Chr1:4548445
   
Insertion allele amplicon 3144 - 18wy
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 18wy: 5'-TCGGTTTCTCTAGATCCTTCCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4547715
   
Insertion allele amplicon 8474 - CW26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW26: 5'-GGAGTGTTGGACTGATACTCCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4549178
   
wt amplicon (over InsPos) CW27 - CW26
wt amplicon start Chr1:4548133
wt amplicon end Chr1:4549178
wt amplicon size 1046 bp
Locus specific primer CW26: 5'-GGAGTGTTGGACTGATACTCCATT-3'
2nd locus specific primer CW27: 5'-CCCTTCACAAACAAACTCTACACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11