Genotyping and primer details


Genotyping and primer details for 066C12 - At3g04290
Line ID 066C12
Locus At3g04290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1134974 of line 066C12 Chr3:1134974
   
Insertion allele amplicon 3144 - 02ew
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 02ew: 5'-GACTGATGCGATCAAATCAACTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1134530
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11