Genotyping and primer details


Genotyping and primer details for 066H06 - At3g49560
Line ID 066H06
Locus At3g49560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18371348 of line 066H06 Chr3:18371348
   
Insertion allele amplicon 8474 - 04jw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04jw: 5'-AAAGAACAGAGAATAGCTCCCACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18371781
   
wt amplicon (over InsPos) 13vg - 04jw
wt amplicon start Chr3:18370730
wt amplicon end Chr3:18371781
wt amplicon size 1052 bp
Locus specific primer 04jw: 5'-AAAGAACAGAGAATAGCTCCCACA-3'
2nd locus specific primer 13vg: 5'-GAATCCAATTCAACAGTTTCAGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11