Genotyping and primer details


Genotyping and primer details for 069H10 - At3g21280
Line ID 069H10
Locus At3g21280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7479016 of line 069H10 Chr3:7479016
   
Insertion allele amplicon 8474 - 07de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07de: 5'-ACCATTGATTATCGCTTTATTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7478633
   
wt amplicon (over InsPos) 07dy - 07de
wt amplicon start Chr3:7478633
wt amplicon end Chr3:7479579
wt amplicon size 947 bp
Locus specific primer 07de: 5'-ACCATTGATTATCGCTTTATTTGC-3'
2nd locus specific primer 07dy: 5'-TAGGGACTGAAAGGAGAACTTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11