Genotyping and primer details


Genotyping and primer details for 070F02 - At3g46370
Line ID 070F02
Locus At3g46370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17052380 of line 070F02 Chr3:17052380
   
Insertion allele amplicon 8474 - KE24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KE24: 5'-CAGACAACGTTGAGATACTTTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17051925
   
wt amplicon (over InsPos) TK50 - KE24
wt amplicon start Chr3:17051925
wt amplicon end Chr3:17052767
wt amplicon size 843 bp
Locus specific primer KE24: 5'-CAGACAACGTTGAGATACTTTCCA-3'
2nd locus specific primer TK50: 5'-AAGCATTTCCCTACTTCACAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11