Genotyping and primer details


Genotyping and primer details for 071E01 - At2g31710
Line ID 071E01
Locus At2g31710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13482433 of line 071E01 Chr2:13482433
   
Insertion allele amplicon 8474 - CD34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD34: 5'-TGTGAGAAAAACAGATCAAATTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13481792
   
wt amplicon (over InsPos) 20sl - CD34
wt amplicon start Chr2:13481792
wt amplicon end Chr2:13482960
wt amplicon size 1169 bp
Locus specific primer CD34: 5'-TGTGAGAAAAACAGATCAAATTGC-3'
2nd locus specific primer 20sl: 5'-TGTATTCTGTTTTCCACATCTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11