Genotyping and primer details


Genotyping and primer details for 072C07 - At1g76460
Line ID 072C07
Locus At1g76460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28687307 of line 072C07 Chr1:28687307
   
Insertion allele amplicon 8474 - 20by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20by: 5'-CTACCAGCAAGGAGTTGTCTATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28687735
   
wt amplicon (over InsPos) 27dn - 20by
wt amplicon start Chr1:28686831
wt amplicon end Chr1:28687735
wt amplicon size 905 bp
Locus specific primer 20by: 5'-CTACCAGCAAGGAGTTGTCTATCC-3'
2nd locus specific primer 27dn: 5'-TGCAAGATAAATAACGTGCAAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11