Genotyping and primer details


Genotyping and primer details for 074A11 - At2g40960
Line ID 074A11
Locus At2g40960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17096791 of line 074A11 Chr2:17096791
   
Insertion allele amplicon 8474 - 05sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05sf: 5'-ATCCCTGTGAAACAGTCAGAAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17096383
   
wt amplicon (over InsPos) 27tf - 05sf
wt amplicon start Chr2:17096383
wt amplicon end Chr2:17097153
wt amplicon size 771 bp
Locus specific primer 05sf: 5'-ATCCCTGTGAAACAGTCAGAAACT-3'
2nd locus specific primer 27tf: 5'-ATTTGTTTACCTGAAAAGGGGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11