Genotyping and primer details


Genotyping and primer details for 076C06 - At5g05540
Line ID 076C06
Locus At5g05540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1638202 of line 076C06 Chr5:1638202
   
Insertion allele amplicon 8474 - 05wj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05wj: 5'-TGGATCATGCCAGAGTAATTGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1637727
   
wt amplicon (over InsPos) 28wt - 05wj
wt amplicon start Chr5:1637727
wt amplicon end Chr5:1638788
wt amplicon size 1062 bp
Locus specific primer 05wj: 5'-TGGATCATGCCAGAGTAATTGATA-3'
2nd locus specific primer 28wt: 5'-TTGCTTTGAGTTCTTGATTTTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11