Genotyping and primer details


Genotyping and primer details for 077A11 - At1g65590
Line ID 077A11
Locus At1g65590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24389615 of line 077A11 Chr1:24389615
   
Insertion allele amplicon 8474 - 03jg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03jg: 5'-CTGTGTTAAGCCTGATTCATCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24390481
   
wt amplicon (over InsPos) 01ur - 03jg
wt amplicon start Chr1:24389170
wt amplicon end Chr1:24390481
wt amplicon size 1312 bp
Locus specific primer 03jg: 5'-CTGTGTTAAGCCTGATTCATCAAA-3'
2nd locus specific primer 01ur: 5'-TATTTGCCACTTCCGGTTATAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11