Genotyping and primer details


Genotyping and primer details for 077H07 - At4g21960
Line ID 077H07
Locus At4g21960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11647395 of line 077H07 Chr4:11647395
   
Insertion allele amplicon 8474 - AH33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AH33: 5'-CAAACCCTTGTTGTCTAGGATGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11646802
   
wt amplicon (over InsPos) AP24 - AH33
wt amplicon start Chr4:11646802
wt amplicon end Chr4:11647939
wt amplicon size 1138 bp
Locus specific primer AH33: 5'-CAAACCCTTGTTGTCTAGGATGTT-3'
2nd locus specific primer AP24: 5'-GATTCGACAAGAAGAGAGCTAGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11