Genotyping and primer details


Genotyping and primer details for 078B06 - At5g13610
Line ID 078B06
Locus At5g13610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4383764 of line 078B06 Chr5:4383764
   
Insertion allele amplicon 8474 - 14bd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14bd: 5'-AATTCTGCTACCATACCATCAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4384266
   
Insertion allele amplicon 8474 - 22ay
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22ay: 5'-CTGAGAGGGCTATCTCTTCGTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4383296
   
wt amplicon (over InsPos) 14bd - 22ay
wt amplicon start Chr5:4383296
wt amplicon end Chr5:4384266
wt amplicon size 971 bp
Locus specific primer 22ay: 5'-CTGAGAGGGCTATCTCTTCGTCTA-3'
2nd locus specific primer 14bd: 5'-AATTCTGCTACCATACCATCAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11