Genotyping and primer details


Genotyping and primer details for 078F06 - At3g05550
Line ID 078F06
Locus At3g05550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1612954 of line 078F06 Chr3:1612954
   
Insertion allele amplicon 8474 - CW64
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW64: 5'-AAGTGCTTCGTACAGTACCATGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1613593
   
wt amplicon (over InsPos) 51vh - CW64
wt amplicon start Chr3:1612518
wt amplicon end Chr3:1613593
wt amplicon size 1076 bp
Locus specific primer CW64: 5'-AAGTGCTTCGTACAGTACCATGTG-3'
2nd locus specific primer 51vh: 5'-TATCCCAAATTTTGATGGTCTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11