Genotyping and primer details


Genotyping and primer details for 079A01 - At1g15670
Line ID 079A01
Locus At1g15670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5390785 of line 079A01 Chr1:5390785
   
Insertion allele amplicon 8474 - 05dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05dl: 5'-GGAAAGCTTCAGGACATAGTCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5390258
   
wt amplicon (over InsPos) 06ec - 05dl
wt amplicon start Chr1:5390258
wt amplicon end Chr1:5391146
wt amplicon size 889 bp
Locus specific primer 05dl: 5'-GGAAAGCTTCAGGACATAGTCAAG-3'
2nd locus specific primer 06ec: 5'-GTTTCCAGTTTTAACCATCGAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11