Genotyping and primer details


Genotyping and primer details for 079C09 - At1g22550
Line ID 079C09
Locus At1g22550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7968086 of line 079C09 Chr1:7968086
   
Insertion allele amplicon 8474 - CD92
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD92: 5'-TTATAGAAGATTCCGTCAGCGACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7968524
   
Insertion allele amplicon 8474 - CD93
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD93: 5'-TAAACCACCACAATAATCGACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7967743
   
wt amplicon (over InsPos) CD93 - CD92
wt amplicon start Chr1:7967743
wt amplicon end Chr1:7968524
wt amplicon size 782 bp
Locus specific primer CD92: 5'-TTATAGAAGATTCCGTCAGCGACT-3'
2nd locus specific primer CD93: 5'-TAAACCACCACAATAATCGACAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11