Genotyping and primer details


Genotyping and primer details for 079F05 - At4g33810
Line ID 079F05
Locus At4g33810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16214538 of line 079F05 Chr4:16214538
   
Insertion allele amplicon 8474 - 03dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03dr: 5'-ATTCATCAAATCGTTTGGATCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16214199
   
wt amplicon (over InsPos) 05es - 03dr
wt amplicon start Chr4:16214199
wt amplicon end Chr4:16214980
wt amplicon size 782 bp
Locus specific primer 03dr: 5'-ATTCATCAAATCGTTTGGATCTTC-3'
2nd locus specific primer 05es: 5'-CAGAGTGATGACAAAGAAGCAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11