Genotyping and primer details


Genotyping and primer details for 081A08 - At5g05840
Line ID 081A08
Locus At5g05840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1757768 of line 081A08 Chr5:1757768
   
Insertion allele amplicon 8474 - 25jd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25jd: 5'-AACCATCTATGGTCCACCACTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1758271
   
wt amplicon (over InsPos) 04jz - 25jd
wt amplicon start Chr5:1757168
wt amplicon end Chr5:1758271
wt amplicon size 1104 bp
Locus specific primer 25jd: 5'-AACCATCTATGGTCCACCACTAAC-3'
2nd locus specific primer 04jz: 5'-ACAGAGGAACTCAAAAACCAAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11