Genotyping and primer details


Genotyping and primer details for 082E03 - At5g64300
Line ID 082E03
Locus At5g64300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25720621 of line 082E03 Chr5:25720621
   
Insertion allele amplicon 8474 - 06ax
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06ax: 5'-TGATTTGTCTTCAGAACATCCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25720116
   
Insertion allele amplicon 3144 - HM53
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer HM53: 5'-GGATTCTACAAGGAATCAACCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25721120
   
Insertion allele amplicon 3144 - MC52
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer MC52: 5'-ATTCTACAAGGAATCAACCAAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25721118
   
wt amplicon (over InsPos) MC52 - 06ax
wt amplicon start Chr5:25720116
wt amplicon end Chr5:25721118
wt amplicon size 1003 bp
Locus specific primer 06ax: 5'-TGATTTGTCTTCAGAACATCCAAG-3'
2nd locus specific primer MC52: 5'-ATTCTACAAGGAATCAACCAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11