Genotyping and primer details


Genotyping and primer details for 083F12 - At2g24230
Line ID 083F12
Locus At2g24230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10303177 of line 083F12 Chr2:10303177
   
Insertion allele amplicon 8474 - KE15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KE15: 5'-TCCAATCATCTGTTGTCTGAACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10302608
   
Insertion allele amplicon 3144 - KE16
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KE16: 5'-AGGATTGAGATGCTTTCTGGTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10303571
   
wt amplicon (over InsPos) KE16 - KE15
wt amplicon start Chr2:10302608
wt amplicon end Chr2:10303571
wt amplicon size 964 bp
Locus specific primer KE15: 5'-TCCAATCATCTGTTGTCTGAACTC-3'
2nd locus specific primer KE16: 5'-AGGATTGAGATGCTTTCTGGTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11