Genotyping and primer details


Genotyping and primer details for 083H10 - At5g01800
Line ID 083H10
Locus At5g01800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:308070 of line 083H10 Chr5:308070
   
Insertion allele amplicon 8474 - 09ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09ra: 5'-AACCATTCGGTAAGAACATTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:307501
   
wt amplicon (over InsPos) 02rj - 09ra
wt amplicon start Chr5:307501
wt amplicon end Chr5:308512
wt amplicon size 1012 bp
Locus specific primer 09ra: 5'-AACCATTCGGTAAGAACATTTTTG-3'
2nd locus specific primer 02rj: 5'-ACTCGAATACCATCTTCTTGCACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11